Join Drs. Tröster and Köhler as they present a novel dura mater transplantation platform for long-term, anesthesia-free imaging of pancreatic islets in awake mice.

Long-term intravital imaging of pancreatic islets is limited by unstable optical access, motion artifacts, and the physiological effects of prolonged anesthesia.

In this webinar, Drs. Philip Tröster and Martin Köhler (Karolinska Institutet) present the dura mater as a novel transplantation and imaging site, enabling stable, longitudinal imaging of pancreatic islets in awake mice. By combining cranial window surgery, islet transplantation, and head fixation in the Mobile HomeCage system, their approach supports repeated, anesthesia-free imaging sessions of up to 90 minutes over several months.

The speakers will demonstrate robust engraftment of mouse and human islets, including rapid vascular integration and preserved metabolic function, and highlight calcium (Ca²⁺) imaging of β-cell activity. The session will also explore advanced microscopy configurations, including 3D imaging, biosensor-based subcellular Ca²⁺ readouts, and near-infrared (NIR/SWIR) imaging.

Discover how this platform opens new possibilities for diabetes research, functional imaging, and long-term intravital microscopy of otherwise inaccessible tissues.

This webinar will be of interest to endocrinologists, neuroscientists, diabetes researchers, intravital microscopy specialists, and scientists seeking innovative solutions for long-term in vivo imaging of transplanted or otherwise inaccessible tissues.

Key Topics:

• Overcoming challenges in long-term intravital imaging of pancreatic islets


• Dura mater as a stable transplantation and imaging site


• Anesthesia-free, longitudinal imaging in awake mice


• Calcium (Ca²⁺) dynamics and β-cell network activity


• Vascular and metabolic integration of transplanted islets


• 3D imaging and biosensor-based subcellular Ca²⁺ monitoring


• NIR/SWIR imaging for deeper tissue penetration