Multiplexing immunofluorescence imaging enables the analysis of the whole tissue microenvironment: the single cell resolution combined with spatial localisation retrieves the information of cellular relationships and organisation within the same morphological context. However, the complexity of the analysis, time-consuming optimisation as well as significant challenges in results reproducibility, slow down the wide adoption of such a powerful research tool. In this talk, we will introduce how the COMET™ performs automated staining and imaging allowing to achieve high-quality, reproducible and uniform results thanks to the Fast Fluidic Exchange™ (FFeX™) microfluidic technology.