Multiplexing immunofluorescence imaging enables the analysis of the whole tissue microenvironment: the single cell resolution combined with spatial localization retrieves the information of cellular relationships and organization within the same morphological context. However, the complexity of the analysis, time-consuming optimization, and execution of staining protocols as well as significant challenges in result reproducibility, slow down the wide adoption of such a powerful research tool. In this talk, we will introduce how LabSat® performs automated staining and allows to achieve high quality, reproducible and uniform results thanks to the Fast Fluidic Exchange™ (FFeX™) microfluidic technology. In the second part, our hyperplex platform Comet™ has been adopted to gather comprehensive insights into various spatial biology research applications such as the biomarker profiling of human prostate cancer tissue by multiplex immunofluorescence assay. Discover how LabSat® and Comet™ can support your research by performing sequential immunofluorescence from healthy to tumoral samples, on mouse and human tissues.