The use of digital PCR for high accuracy molecular measurement
Digital PCR (dPCR) counts DNA molecules, separated by limiting dilution in a defined volume, providing high accuracy estimation of copy number. The method offers high reproducibility, improved sensitivity when measuring rare genetic variants, and greater confidence for low level, trace analysis. This presentation will introduce dPCR, discuss its advantages, and outline how the approach might be used to improve measurement in areas like clinical diagnosis, alone or in conjunction with other methods.
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