Automated Chromogenic RNAscope™ In Situ Hybridization on LabSat® Research
The RNAscope™ assay, based on ACD’s technology for signal amplification and background suppression, was optimized on LabSat® to result in well-preserved morphology, uniform staining with excellent signal-to-noise ratio, and readily detectable punctate signal dots – comparable to the manual assay on mouse and human FFPE samples. Concomitantly, the assay turnaround time was shortened with respect to the manual and other automated CISH protocols. Overall, we have proven the capability of the microfluidic platform LabSat® to automate the RNAscope™2.5 HD-BROWN CISH assay with reproducible staining results. The microfluidic platform brings the benefits of a shorter run time and reliability of a compact desktop system.
Lunaphore microfluidic technology for tissue analytics
LabSat® Research staining plarform
RNAscope™ technology and general workflow
RNAscope™ automation and workflow on LabSat® Research
Results: control and target probes on cytological/histological FFPE samples