Webinar: Understanding Advanced Cardiac Tissue Slice Applications by InsideScientific

Understanding Advanced Cardiac Tissue Slice Applications

About

Watch to understand how cardiac slices and the IonOptix Cardiac Slice System can be used to complement and improve upon traditional cardiac investigations.

Cardiac slices better preserve the structure, function, and biochemical properties of the in situ heart when compared to other model systems, including isolated cardiac myocytes and other intact tissue preparations, while allowing for a wider range of experimentation than whole heart. Additionally, cardiac slices can be prepared from both animal and human tissue, suggesting that data will translate better from the bench to the clinic.

In this webinar, the speakers build off the previous IonOptix webinar introducing the cardiac slice preparation to explore specific applications that are well-suited to cardiac slices and the IonOptix Cardiac Slice System. Specifically, they show investigations utilizing cardiac slices to highlight the effect of myosin ATPase inhibition on the Frank-Starling relationship, as well as the relationship between microtubule network remodeling and diastolic stiffness/dysfunction. Lastly, they also explore engineered heart tissue as an alternative to the cardiac slice preparation.

Key Topics:

Acquiring work loops in cardiac slices

Visualizing the Frank-Starling relationship in cardiac slices

Using cardiac slices to understand the molecular mechanisms responsible for cardiac stiffening

How to acquire work loops in engineered heart tissue

Presenters

Bradley Palmer, PhD

Assistant Professor of Molecular Physiology and Biophysics, University of Vermont

Dr. Bradley Palmer's professional activities have focused on original research demonstrating the molecular basis of cardiac muscle relaxation function. His focus is on two areas: myofilament basis of muscle dynamic stiffness and zinc-dependent alterations in calcium regulation.

Matthew Caporizzo, PhD

Assistant Professor of Molecular Physiology and Biophysics, University of Vermont

Dr. Matthew Caporizzo's Lab studies the mechanics of heart failure with particular emphasis on understanding the molecular mechanisms that stiffen the heart and how the heart's stiffness influences its function in health and heart disease.

Benjamin Lee, MD, PhD

Fellow, Cardiovascular Medicine, University of Pennsylvania

Dr. Benjamin Lee is currently a cardiology fellow at the University of Pennsylvania. He received his MD/PhD from Columbia University in biomedical engineering. He is fascinated by beating heart tissues and is using 3-dimensional systems to better understand and target mechanical dysfunction in the heart.

First Name*

Last Name*

Email Address*

Organization / Company*

Job Title*

Country / Region*

Which cell/tissue types do you use in your research (select all that apply)? *

Adult isolated (single) cardiomyocytes

Neonatal cardiomyocytes

ESC- or iPSC-cardiomyocytes

Cardiac tissue (papillary, trabeculae, slices, etc.)

Whole heart

Engineered tissue

Other

Which of the following methods/techniques do you currently use with cardiac slices and engineered tissue (select all that apply)?

Force measurements

Calcium, membrane potential, etc. with fluorescent indicators

Mechanical work and/or compliance/stiffness measurements

Long-term remodeling

Other

None

What is your greatest challenge as it relates to cardiac physiology?

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