About
Reproducible research with human induced pluripotent stem cells (iPSCs) depends on thoroughly characterized and quality-controlled cell lines. In this webinar, Dr. Andrew Gaffney and Dr. Erin Knock from STEMCELL Technologies describe the generation of a standardized induced pluripotent stem cell (iPSC) line. Developed with the upcoming ISSCR Standards Initiative characterization guidelines in mind, this highly characterized line is karyotypically stable, demonstrates trilineage differentiation potential, and expresses undifferentiated cell markers. Further, STEMCELL has developed a highly pure, ready-to-use neural progenitor cell product expressing PAX6 and SOX1 over multiple passages.

Dr. Knock shaws how these multipotent cells are suitable for customized downstream differentiation to various CNS cell types, such as forebrain neurons, midbrain neurons, and astrocytes. These progenitor cells are the ideal controls for standardizing downstream differentiation protocols, modeling diseases, and assay development.

Key learning objectives:
  • Discover how STEMCELL’s induced pluripotent stem cell lines are derived and characterized

  • Learn how to differentiate induced pluripotent stem cell lines into all three germ layers

  • Explore the features of STEMCELL’s neural progenitor cell product

  • Differentiate neural progenitor cells into a variety of neural cell types, including neurons and glia
Presenters
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Andrew Gaffney, PhD
Director, Stem Cell Manufacturing and Commercialization, STEMCELL Technologies
Dr. Andrew Gaffney is the Director of Stem Cell Manufacturing and Commercialization at STEMCELL Technologies, where he oversees the development of human iPSCs, differentiated cells, and organoids. Andrew completed his PhD in pediatric oncology at the University of Leeds where he successfully established a human embryonic stem cell disease model of Ewing sarcoma. After joining STEMCELL in 2014, Andrew managed the company's pluripotent stem cell product portfolio. He has also developed strategic alliances with stem cell scientists in academia and industry to support the development of PSC-based disease models, drug screens, and cell therapy applications.
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Erin Knock, PhD
Associate Director, Neural Biology, STEMCELL Technologies
Dr. Erin Knock is the Associate Director of Neural Biology in the Research and Development Department at STEMCELL Technologies and an Adjunct Professor of Biology at Simon Fraser University. She obtained her PhD in Human Genetics from McGill University in 2009. She was a postdoctoral fellow at the University of Cambridge from 2009-2012, where she worked on neural stem cell differentiation during development. In 2012, she relocated to the University of Toronto to develop human stem cell-derived neuronal models of Alzheimer’s Disease. In 2016, Erin was recruited to STEMCELL Technologies where she oversees the development of products for primary and stem cell-derived neural cell culture. Erin and the Neuroscience Group are proud to support products for both primary neural culture and pluripotent stem cell differentiation, including organoid differentiation.
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