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Please note: due to unforeseen circumstances, this webinar has been temporarily postponed. We will be rescheduling the event, and you will receive further information when the new date has been finalized.

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In this webinar, Dr. Jonathan Brestoff discusses his research on metabolism and a new method to quantify metabolic data known as Clambake.

Changes in energy expenditure are observed in a wide variety of disease and physiologic states. The most frequently used method to estimate energy expenditure in small animal models is indirect calorimetry, which is utilized in metabolic cage systems. However, total energy expenditure is a composite of four major parameters: basal metabolic rate, activity-induced energy expenditure, the thermic effect of food, and adaptive thermogenesis. This presentation describes a new bioinformatic approach called Clambake that estimates these four parameters using metabolic cage data. Clambake is sufficiently robust to analyze data from new experimental conditions and can reveal hidden phenotypes that might otherwise be overlooked. Therefore, Clambake provides an unprecedented level of detail in metabolic cage data and has the potential to enable novel insights about energy homeostasis in mouse models of physiology or disease.

Key Topics:

  • To learn how metabolic cage systems function and their value in studying health and disease

  • To identify the 4 major parameters that make up total energy expenditure

  • To understand the basis of Clambake and the ability of this method to estimate the 4 major energy expenditure parameters using metabolic cage data

When
Friday, June 14, 2024 · 11:00 a.m. Eastern Time (US & Canada) (GMT -4:00)
Presenter
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Jonathan Brestoff, MD, PhD, MPH
Assistant Professor, Department of Pathology and Immunology, Washington University School of Medicine
Dr. Brestoff received his MD/PhD from University of Pennsylvania with Dr. David Artis, where his research identified a role for Group 2 innate lymphoid cells (ILC2s) in promoting beige fat biogenesis (Brestoff et al., Nature, 2015; Brestoff & Artis, Cell, 2015). He recompleted residency in Clinical Pathology at Washington University School of Medicine, during which time he pioneered studies with Dr. Steven L. Teitelbaum and later in his own lab on cell-to-cell transfer of mitochondria in regulating energy homeostasis (Brestoff et al., Cell Metabolism, 2021; Borcherding/Jia et al., Cell Metabolism, In Press). As a recipient of the NIH Director’s Early Independence Award and Burroughs Wellcome Fund Career Scientists Award, Dr. Brestoff started his independent laboratory at WashU as Assistant Professor of Pathology and Immunology in 2019, leading a research program studying intercellular mitochondria transfer and how the immune system regulates metabolic adaptation to nutrient stress.
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