About
Spatial Signatures are a new class of highly predictive biomarkers that measure the interactions and cellular densities of tumor and immune cells that compose the tumor microenvironment. Based on multiplex immunofluorescence, spatial signatures provide a deeper understanding of complex interactions between tumors and the immune system, enabling improved patient stratification for immunotherapies. A significant hurdle to date has been in developing a data analysis workflow that is straightforward and user-friendly to transform the data rich images into meaningful quantitative spatial signatures.

In this webinar, Aditya and Lorcan will review the key features of the new PhenoImager HT 2.0 data analysis workflow. This workflow introduces a simplified framework from scanning to analyzing spectrally unmixed multiplex immunofluorescence images generated on the PhenoImager HT platform. The ready-to-analyze data can be directly imported into image analysis software such as Visiopharm. This presentation will cover key aspects of data analysis elements such as image QC, segmentation, phenotyping, and verification – all essential for creating outputs that support the development of spatial signatures.

Key learning objectives will include:
  • Understand Akoya’s new HT 2.0 data analysis workflow

  • The challenges in multiplex immunofluorescence analysis and the use of AI and cell lineage segmentation considerations

  • Explore OracleBio’s image analysis workflow incorporating Visiopharm

  • Evaluation of analysis data to facilitate spatial profiling and interpretation
Register To Watch Recording
First Name*
Last Name*
Email Address*
Organization / Company*
Job Title*
Country / Region*
Postal Code*
Do you own or have access to any of the following instruments?*
PhenoCycler-Fusion or PhenoCycler (formerly, CODEX)
PhenoOmager Fusion or PhenoImager HT (formerly, Vectra Polaris)
Both the PhenoCycler and PhenoImager Platforms
None of the above
What analyte type or panel composition would you be interested in adopting?*
Protein markers only
Primarily protein with a handful of RNA markers
Primarily RNA with a handful of protein markers
RNA markers only
Not sure yet
None of the above
Have you factored in analysis of multiplex images to your workflow?*
Yes
No
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