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Analysis of Peripheral Blood Mononuclear Cells from Recovered COVID-19 Subjects

About This Webinar

The discovery of durable memory B cell (MBC) subsets targeting protective viral epitopes is critical for determining immune correlates of protection from SARS-CoV-2 infection. Here, we identified functionally distinct SARS-CoV-2-specific B cell subsets by profiling the repertoire of convalescent COVID-19 patients using a high-throughput B cell sorting and sequencing platform. Utilizing distinct barcoded SARS-CoV-2 antigen baits, we isolated thousands of B cells reactive to the spike protein, spike receptor binding domain, nucleoprotein (NP), and open reading frame proteins (ORF) 7 and 8. Spike-reactive B cells were enriched in canonical MBC clusters even in subjects with low serum titers, and monoclonal antibodies (mAbs) from these cells were potently neutralizing. B cells reactive to ORF8 and NP were enriched in naïve-like clusters and mAbs against these targets were exclusively non-neutralizing. Moreover, our approach offers a high-throughput method for the identification of mAbs reactive to distinct SARS-CoV-2 antigens.

Who can view: Everyone
Webinar Price: Free
Featured Presenters
Webinar hosting presenter
Graduate Student, Research Assistant, University of Chicago
Chris is a graduate student in the Patrick Wilson Laboratory at the University of Chicago. His research has focused on the mechanisms that drive somatic hypermutation and their impact on vaccination strategies. His research has used a combination of traditional immunology techniques combined with modern, high throughout approaches. He has previously worked on understanding B cell and antibody responses to a chimeric-hemagglutinin influenza vaccine, and currently studying the same responses to SARS-CoV-2, the causative agent of COVID-19.
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