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A Synthetic Biology Approach to Enzyme Engineering

May 17, 2017 at 02:00:00 PM · Eastern Time (US & Canada)
About This Webinar

"Effective mutagenesis strategies in enzyme engineering are often dependent on the generation of small and targeted, high-quality libraries of mutants. Such 'smart' libraries are consistent with practical constraints imposed by the screening effort; while point-mutant libraries are readily screened, we need creative solutions to improve our capacity to explore the combinatorial complexity of sequence space."

In this webinar, Dr. Quaglia will talk about how she used a flexible approach based on directed evolution and the Golden Gate cloning strategy to target three distinct regions of Candida antarctica lipase A (Cal-A) generating a series of diverse libraries. Screening to improve discrimination of short-chain versus long-chain fatty acid substrates was aided by the development of a general, automated method that allowed for visual discrimination in the hydrolysis of varied substrates in whole cells.

Categories:
SCIENCE & TECH
Who can view: Everyone
Webinar Price: Free
Webinar ID: d8230789e7b3
Featured Presenters
Webinar hosting presenter GENEWIZ
GENEWIZ is a global leader in genomics services that enable research scientists within pharmaceutical, biotechnology, agriculture, environmental and clean energy, academic, and government institutions to advance their discoveries. Customers rely...
Webinar hosting presenter Daniela Quaglia
Postdoctoral Researcher in Biocatalysis at Université de Montréal
Daniela Quaglia, Ph.D., is a Postdoctoral Researcher in Biocatalysis at Université de Montréal in the laboratory of Prof. Joelle Pelletier. Her current project (co-funded by NSERC and the industrial partner DSM) focuses on the use of enzyme...
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